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Published 11:04 11 Sept 2016 BST
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However, having sustained a fractured eye socket in the second round, the Briton struggled with blurred vision and Golovkin gradually began to dominate.
With the shots raining down on Brook in round five, the 30-year-old's trainer Dominic Ingle decided the time was right to throw in the towel.
While many saluted Brook's bravery in the immediate aftermath of the fight, Chris Eubank Jr took a different approach.
In a tweet to Golovkin, the 26-year-old made it clear that he'd be willing to be his next opponent, suggesting he was a 'real' British middleweight and that his corner wouldn't be throwing in any towels.
https://twitter.com/ChrisEubankJr/status/774738105853177856
The tweet drew plenty of criticism from those on Twitter, with Barry Hearn - father of Brook's promoter, Eddie - describing it as 'shameful'.
https://twitter.com/BarryHearn/status/774758035566755840
Others were also quick to criticise the tweet, with many pointing out that Brook had taken the fight on after Eubank Jr had appeared to turn turn down the chance to fight GGG.
Earlier in the year, Golovkin claimed on Twitter that he had agreed to face Eubank Jr, and suggested that the Brit had reservations about facing him.
'Everything is agreed from our side,' he tweeted. 'Now waiting for @ChrisEubankJr, who all of a sudden has lost his pen.'
https://twitter.com/ElliotSmith75/status/774766315324248064
https://twitter.com/MartinJessopp/status/774738351496826880
https://twitter.com/Greenzeroboxing/status/774738751398539264
https://twitter.com/FrankCraig13/status/774738493478203392
https://twitter.com/PapaBeats/status/774738968575406080

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